Figure 5
Live imaging after IR irradiation
(A and B) Left, image showing the IR laser target area (17 μm radius circle in the middle of the telencephalon) at 12 hpf and Right, imaging over time from 12 hpf to 24 hpf in a (A)
(C and D) Plot profiles of mCherry-Cxcl12a expression (Gray value) visualized in (A-B, dashed white rectangle) respectively using Fiji.
(E) Visualization of mCherry-Cxcl12a activation at 16 hpf using the Imaris “Surface” tool. The picture in