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Fig. 4
Zebrafish rerea homozygous mutants exhibit disruption of retinal optic stalk boundary. Zebrafish larvae homozygous for rerea mutation exhibit disruption of retinal optic stalk boundary (A–C, D–F), which was further confirmed by isolation of eye cups (A, D, bottom panels) and detailed coronal histological sectioning (B, E) of 6 dpf larvae. Unfused margins of optic fissure can be observed (D bottom panel, red arrow). The presence of ectopic differentiated retinal layers (C, F) was confirmed by immunostaining with ZPR-1 (photoreceptors) and HuC/D (amacrine cells and ganglion cells).
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