Figure 3

Figure 3

Midbrain and rHb GFP+ cells do not express other monoaminergic/catecholaminergic markers. (A–C”) Whole brain fluorescent in situ hybridization for the indicated mRNAs (magenta) and immunofluorescence staining for GFP (green) in th^{m1512Tg(2A−QF2)}; Tg(QUASr:GFP)c403 larval brains at 120 hpf. Dorsal views of z-projections. Anterior is to the left. (A–A”) Expression of the dopaminergic marker slc6a3 in comparison with GFP expression in the pretectum, midbrain, and hindbrain. (B–B”) Expression of the monoaminergic marker slc18a2 in comparison with GFP expression in the pretectum, midbrain, and hindbrain. (C–C”) Expression of the catecholaminergic marker ddc in comparison with GFP expression in the pretectum, midbrain, and hindbrain. Scale bar: 100 μm. For better representation of low and high signal intensities, non-linear adjustments were made to whole image panels (see Section 2.8). LC, locus coeruleus; MHB, midbrain–hindbrain boundary; Mb, midbrain; Pr, pretectum; rHb, rostral hindbrain; RN, raphe nucleus.