Figure 3-supplement 1.

Figure 3-supplement 1. Representative genes expressed in the major resident macrophage clusters Mac 2 and Mac 3 of infarcted heart.

(A) Heatmaps shows the top DEGs enriched in Mac 2 and Mac 3. The color represents the gene expression level in average log2 fold change. (B) Expression of hbaa1 (Mac 2) and timp4.3 (Mac 3) was detected (arrows and arrowheads) by chromogenic in situ hybridization at 7 dpci. hbaa1 was also expressed in RBC (stars in the left lower panel). Dotted lines delineate injury areas (upper panels); scale bars, 50 μm (upper panels) and 10 μm (lower panels). (C) UMAP plot of the monocyte-derived/circulating macrophage marker gene ccr2 (left panel). Dot plot shows the ccr2 expression in different macrophage clusters (right panel). The circle size represents the percentage of macrophages expressing the gene, whereas the color represents the expression level. DEGs, differentially expressed genes; M, macrophage; N, neutrophil; MN, hybrid; Throm, thrombocyte; DC, dendritic cell; FB, fibroblast; R, red blood cell; UMAP, Uniform Manifold Approximation and Projection.