Fig. 2

Fig. 2

Fig. 2. Dhx15 is essential for zebrafish intestinal development. (A-E A'-E') WISH analysis of fabp2 (A A'), apoa1a (B B′), apoa4b.1 (C C′), aqp3a (D D′), fabp6 (E E') expression in zebrafish embryos at 3.5 dpf. The expression of fabp2 (expressed in small intestinal epithelial cells), apoa1a, apoa4b.1 (expressed in the gut), aqp3a (expressed in the gastrointestinal tract), and fabp6 (expressed in midgut) were dramatically deceased in dhx15 knockout compared with the wide-type zebrafish. (F-J F′-J') WISH analysis of fabp2 (F F′), apoa1a (G G'), apoa4b.1 (H H′), aqp3a (I I′), fabp6 (J J') expression in zebrafish embryos at 60 hpf. The expression of fabp2, apoa4a, apoa4b.1, aqp3a, and fabp6 were marginal decreased in dhx15 knockout compared with the wide-type zebrafish. (K-O K′-O′) WISH analysis of fabp2, apoa4a, apoa4b.1, aqp3a, fabp6 expression in zebrafish at 3.5 dpf. The expression of fabp2, apoa4a, apoa4b.1, aqp3a, and fabp6 in dhx15^−/−;Tg (hsp70:DHX15-2A-mcherry) zebrafish were restored (K′-O′) after overexpressing DHX15 by conditional heat activation. The arrows indicate the intestine of zebrafish.