ZFIN Image: Ma et al., 2023, Fig. 3

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Figure Caption

Fig. 3

Oxoeicosanoid and not LTB₄ signaling regulates early neutrophil recruitment along with microbial pattern recognition

(A and B) Myd88 and pycard mutations (F0 CRISPR) inhibit early neutrophil recruitment to infected ears to a similar extent as hcar1–4^{mk214 −/−} mutation does but without having an additive effect to the latter.

(C and D) Unlike the hcar1–4^{mk214 −/−} mutation, lta4h and ltb4r F0 CRISPR mutations do not inhibit early neutrophil recruitment to infected ears. Violin plot points denote counts of Sudan-black-stained neutrophils in individual larvae. Violin plots lines denote first quartiles (top line), medians (middle line), and third quartiles (bottom line). **p < 0.01; ***p < 0.001; ****p < 0.0001; n.s., p > 0.05; Student’s t test (two tailed, two sample unequal variance test). Parentheses, total number of injected larvae.