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Fig. 2

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ZDB-IMAGE-230707-53
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Figures for Zhang et al., 2023
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Fig. 2 Wdr5 promotes the differentiation of digestive organs through H3K4me3, but not H4K16ac.

a Western blot of H3K4me3 in WT and wdr5−/− mutant embryos at 3 dpf. Relative intensity of H3K4me3 was normalized with H3. b Western blot of H4K16ac in WT and wdr5−/− mutant embryos at 3 dpf. Relative intensity of H4K16ac was normalized with H3. c Cryosections of WT and wdr5/− mutant embryos at 3 dpf were immunostained by anti-H3K4me3 (in red). The nuclear was stained with DAPI (in blue). L: liver; I: intestine; P: pancreas. Scale bar: 40 μm. d Venn diagram showing 953 overlapping downregulated genes between RNA-seq (wdr5−/− mutant vs WT, log2FoldChange ≤ −1, Padj < 0.05) and H3K4me3 ChIP-seq (wdr5−/− mutant vs WT, log2FoldChange ≤ −0.58, Padj < 0.05). e KEGG analysis of overlapping downregulated genes between RNA-seq and H3K4me3 ChIP-seq in d. f Distribution of H3K4me3 peak changes (wdr5−/− mutant vs WT, |log2FoldChange | ≥ 0.58, Padj < 0.05) in 239 downregulated genes (wdr5−/− mutant vs WT, log2FoldChange ≤ −1, Padj < 0.05) enriched in digestive organs such as liver, intestine and exocrine pancreas. g Graphs showing H3K4me3 and RNA peaks at apom and ebp loci in WT and wdr5−/− mutant embryos at 3 dpf. h WISH of apom and ebp in WT and wdr5−/ mutant at 3 dpf. Each experiment was repeated for three times with similar results and a representative was showed here. n indicates the number of zebrafish embryos in each group. Data are mean ± S.D. Two-tailed t-test was applied for each individual comparison (**p < 0.01; n.s no significance).

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