Figure 6

Figure 6

BIBR treatment downregulated the expression of zebrafish myca and mycb and upregulated p21. Twelve hours post fertilization (hpf), zebrafish wild-type (WT) and tert mutant (tert^{hu3430/hu3430}; tert −/−) embryos were treated with 2 μM BIBR or DMSO as a control for 12 h, from 12 to 24 hpf. Levels of relative mRNA expression for the indicated genes in WT (A–C) and tert mutant (D–F) embryos are shown. Data represent the mean and SD (bar) from three separate experiments. (G) Representative Western blots showing total protein levels of Myc in WT and tert mutant zebrafish embryos upon treatment with 2 μM BIBR for 12 h. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as a loading control. The original Western blots are shown in File S1. The graph shows the values in arbitrary units of densitometric analysis performed with ImageJ software. Data represent the mean and SD (bar) from two separate experiments. A significant difference between values in BIBR-treated embryos vs. DMSO-treated embryos is shown: * p < 0.05; *** p < 0.001; ns: not significant.