FIGURE 5

FIGURE 5

Expression of the Et(fzd1:EGFP) line in the developing inner ear. (A–C′) Images of the otic vesicle of Et(fzd1:EGFP) embryos taken with a Nikon CSU‐W1 spinning disk confocal microscope at 3 dpf; lateral views with anterior to the left. All are single z‐slice images taken at different focal planes. (A–C) Inverted images of GFP expression. (A'–C′) Merged channel images showing Et(fzd1:EGFP) expression in green and Tg(xEF1A:H2B‐RFP) expression in all nuclei in magenta. (A, A') Lateral view of the ventral pillar, marked by expression of GFP. (B, B′) GFP expression in the three cristae (B, asterisks) is strongest in the supporting cells at the margins of the lateral crista (B, arrowheads; compare to the fzd1 mRNA expression in Figure 6f, inset). (C, C′) Dorsal view of the head at the level of the ventral pillar (C, arrows mark expression in the ventral pillars; compare to the fzd1 mRNA expression in Figure 6e). GFP is also expressed in the lens of the eye, epiphysis (C, arrowhead) and pectoral fins. Abbreviations in C: ov, otic vesicle; pf, pectoral fin. Scale bars: 20 μm in (A'), for (A); 20 μm in (B′), for (B); 100 μm in (C′) for (C).