FIGURE 1

FIGURE 1

Expression of the Et(smad6b: EGFP‐CAAX) line. (A–C′) Whole mount images of Et(smad6b:EGFP‐CAAX); Tg(xEF1A:H2B‐RFP) double transgenic embryos taken with a Nikon CSU‐W1 spinning disk confocal microscope at 1, 2 and 3 days post fertilisation (dpf); lateral views, anterior to the left. Arrowhead in (A) marks the otic vesicle. Panels (a–c') show maximum intensity projection (MaxIP) enlargements of EGFP expression in the otic vesicle at 1–3 dpf, corresponding to the white boxes in (A'–C′). Strong expression is present in the three cristae (b, asterisks). EGFP expression is also seen in the pharyngeal region at 24 hpf (a, arrowhead) and in the developing pectoral fin (B, arrowhead) at all stages. Lower level expression is present throughout the skin at all stages and in the forming opercle and cleithrum at 3 dpf (C, arrowheads). The Tg(xEF1A: H2B‐RFP) line provides a counterstain for all nuclei (magenta). Scale bars: 500 μm in (A'), for (A–C′); 50 μm in (a') for (a–c').