ZFIN Image: Bai et al., 2023, FIGURE 2

Image

Figure Caption

FIGURE 2

The expression of jag1a or jag1b was effectively knocked down with MO. (A) The target sites of jag1a-SBMO and jag1b-SBMO are at I5E6 and I2E3, respectively. The target sites of jag1a-TBMO and jag1b-TBMO are both located in E1. (B, C) RT-PCR confirmed the effectiveness of jag1a-SBMO and jag1b-SBMO, which demonstrated by the appearance of abnormally spliced fragments marked by red arrows. Abbreviations: SBMO, splice-blocking morpholino; TBMO, translation-blocking morpholino; E, exon; I, intron; RT-PCR, reverse transcription-polymerase chain reaction.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Genet