Fig. 2.

Fig. 2.

eng^mu130 mutant embryos have increased basilar artery diameter and increased number of endothelial kugeln. (A) Representative maximum-intensity projection of Tg(kdrl:Hsa.HRAS-mCherry)^s916 zebrafish brain vasculature at 72 hpf. Black arrowheads indicate individual kugeln. Scale bars: 150 μm/50 μm (inset). Three-dimensional reconstruction of the same image shown in green is on the right. (B) Representative maximum-intensity projection of the basilar artery (BA) at 72 hpf. Black arrowheads indicate individual kugeln. White bars indicate where diameter of the BA was measured. Scale bar: 50 μm. Three-dimensional reconstructions of the same images shown in green are on the right. (C) BA diameter in eng^mu130 mutants and WT siblings (unpaired two-tailed Student's t-test, 10-12 animals/group). (D) eng mutation did not alter endothelial cell (EC) number in the BA (unpaired two-tailed Student's t-test, 10-12 animals/group). (E) Kugel number per animal was increased in eng^mu130 mutants at 52 and 74 h post fertilisation (hpf) but not at 100 hpf. *P<0.05; ns, not significant (Mann–Whitney U-test, 8-27 animals/group).