ZFIN Image: Peterman et al., 2023, Fig. 4.

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Figure Caption

Fig. 4.

Langerhans cells represent the mpeg1⁺ cell type that engulfs cutaneous axon debris. (A) Schematic (left) and corresponding fluorescent image (right) comparing the morphology of a Langerhans cell (arrow) and a metaphocyte (arrowhead). (B) Quantification of axonal debris engulfed relative to number of protrusions in mpeg1⁺ cells 60 min post-scale pluck-induced axon degeneration, n=12 scales from N=9 fish. (C) Fluorescence in situ hybridization with HCR probes against cd4-1 following scale pluck-induced axon degeneration. Arrows indicate cd4-1 expression in mpeg1⁺ cell; arrowheads indicate engulfed axonal debris. (D) Fluorescence images of the scale epidermis in an adult expressing Tg(mpeg1:NTR-EYFP), Tg(mfap4:tdTom-CAAX) and a somatosensory axon reporter [Tg(p2rx3a:mCherry)]. Arrows indicate mpeg1⁺/mfap4⁺ cells (Langerhans cells); arrowheads indicate an mpeg1⁺ only cell (metaphocyte). (E,F) Still images of the scale epidermis in a Tg(mpeg1:NTR-EYFP);Tg(mfap4:tdTom-CAAX);Tg(p2rx3a:mCherry) adult before and during scale pluck-induced axon degeneration. Arrowheads indicate engulfed axonal debris. Time denotes mm:ss. Scale bars: 5 μm (A), 10 μm (C-F). Error bars in B denote s.d.

Acknowledgments

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