|
FIGURE 11
Number of postsynaptic receptors are reduced in ethanol-exposed fish (A–F) Immunostained neuromuscular junctions (NMJs) composed of presynaptic terminals (labeled with SV2) and postsynaptic acetylcholine receptors (labeled with α-BTX) in untreated (A–C) and ethanol-exposed (D–F)isl1:RFP fish. A and D are merged images with motor neurons (in blue), SV2 (in green) and α-BTX (in magenta). (G–R) Quantification of presynaptic and postsynaptic terminals in untreated (G–L) and ethanol-exposed (M–R) fish. NMJs were counted for Intermandibularis anterior, Intermandibularis posterior and Interhyal muscles by selecting an area of 300x180 um^2 as shown in G, J, M and P. H, K, N and Q show total number of particles after segmentation. I, L, O and R show particles with sizes greater than 8-micron square. (S) Number of α-BTX labeled particles >8 um^2 significantly reduced in ethanol-exposed fish. There was no significant change in the number of SV2 labeled particles. * = p < 0.05; ** = p < 0.01. Scale bar = 100 um.