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FIGURE 7
Ethanol induces cranial nerve defasciculation (A, B) Untreated and ethanol-exposed sox10:Kaede;isl1:RFP fish. Scale bar = 100 um. (C, D) Magnified view of motor innervations in Intermandibularis anterior (M.IMA), Intermandibularis posterior (M.IMP) and Interhyoideus muscles (M.IH) in untreated and ethanol-exposed fish. Scale bar = 50 um. Arrow in D indicates defasciculated exons around MHJ (mylohyoid junction). (E, F) High-resolution images of MHJ region in untreated and ethanol-exposed fish. (G) Number of fascicles at MHJ significantly increase in fish exposed to 1% ethanol.* = p < 0.05; *** = p < 0.001; **** = p < 0.0001.