ZFIN Image: Ritter et al., 2023, Figure 2

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Figure Caption

Figure 2

Rab6a vesicle trafficking is reduced in rgp1^-/- chondrocytes (A) Experimental design for mosaic overexpression of WT mCherry-zRab6a fusion protein in Tg(Col2a1α:caax-eGFP) transgenic zebrafish chondrocytes for live imaging. (B) Representative images from movies of mCherry-zRab6a vesicular compartment movement in chondrocytes ( Supplemental Movies 1, 2). (C) Montage of mCherry-zRab6a puncta progression at specific times in WT and rgp1^-/- chondrocytes (yellow arrowheads) showing path of indicated vesicle over time (white line). (D) Quantification of vesicular compartment distances traveled in WT and rgp1^-/- chondrocytes. Red lines indicate means. (E) Quantification of vesicular compartment speeds in WT and rgp1^-/- chondrocytes. Red lines indicate means. Significance is presented by ****p<0.0001.

Acknowledgments

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