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Figure 6

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ZDB-IMAGE-230211-101
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Figures for Tonelli et al., 2023
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Figure 6

Osteoclast analysis during caudal fin regeneration in tmem38b mutants. (A) Representative images of TRAP staining of caudal fins of WT and tmem38b mutants at 5 dpa (WT n = 7, tmem38b-/- n = 9, tmem38bΔ120-7/Δ120-7 n = 10). In WT, osteoclasts were present in the regenerate. Almost no TRAP activity could be detected in tmem38b-/- , while in tmem38bΔ120-7/Δ120-7 it seemed to be mostly localized along the regenerating fin rays. TRAP+ cells were significantly reduced in tmem38b-/- respect to the other two groups. (B) Relative expression of bone resorption-related markers acp5a and ctsk, of the macrophage marker mpeg1 and rankl/opg ratio in amputated and 5 dpa caudal fin (WT n = 3, tmem38b-/- n = 3, tmem38bΔ120-7/Δ120-7 n = 3). All markers were increased after amputation. mpeg1 was significantly overexpressed in tmem38bΔ120-7/Δ120-7 compared to WT at 5 dpa.*: p < 0.05. (C) Representative images of picro sirius red staining of actinotrichia in caudal fins of WT and tmem38b mutants (WT n = 3, tmem38b-/- n = 3, tmem38bΔ120-7/Δ120-7 n = 3). tmem38b-/- revealed a reduced number of actinotrichia respect to WT and tmem38bΔ120-7/Δ120-7 , while the length of actinotrichia was reduced in both mutants compared to WT. *p < 0.05.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)