ZFIN Image: Morales-Curiel et al., 2022, Fig. 7

Image

Figure Caption

Fig. 7

3D calcium imaging of freely moving animals.

a Sequence of reconstructed 3D images of a moving animal showing high-calcium activity at its contracted side. Images show a single plane of the reconstructed z-stack. Inset corresponds to the raw lightfield image. Scale bars = 40 μm. b Sideview image of the curvature-dependent calcium signal in muscles during ventral and dorsal body bends with warmer colors representing higher calcium signals. Scale bar = 20 μm. The polar plot shows the intensity distribution on the ventral and dorsal side. Dotted line corresponds to the circumference of the animal. c Intensity of the bioluminescent calcium indicator and curvature variation on the ventral side during animal crawling under the lightfield microscope. Black = curvature, yellow = calcium signal.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Commun Biol