ZFIN Image: Morales-Curiel et al., 2022, Fig. 5

Image

Figure Caption

Fig. 5

Seamless denoising and segmentation of bioluminescent zebrafish embryos and mouse embryonic stem cells.

a Laser scanning confocal fluorescence image of a 4 h post-fertilization (hpf) zebrafish embryo expressing membrane-bound GPI-GFP taken on a Leica SP5. The red box indicates the close-up below. Scale bar = 50 μm. b Unprocessed bioluminescence image of a 4 hpf zebrafish embryo expressing GPI-GFP targeted to the plasma membrane. The red square indicates the high magnification close-up below. Scale bar = 100 μm. c The same bioluminescent signal of the zebrafish embryo was restored using a pretrained CARE pipeline optimized for epithelial monolayers. The bottom picture shows the segmented bioluminescent image. No segmentation was possible on the raw image. Scale bar = 20 μm. d Brightfield (i) and fluorescence image (ii) of a spheroid of mouse embryonic stem cells. e Unprocessed raw (i) and denoised (ii) bioluminescence image of similar spheroids. Scale bar = 75 μm. f Segmented nuclei (i) after denoising, overlayed with their individual tracks throughout the timelapse (ii). Scale bar = 35 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Commun Biol