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Fig. 2
Stress reporter activation under external illumination and heat.
a, Sketch of the bioluminescent reporter. the full-length daf-16 cDNA is fused to a mNeonGreen fluorophore and a NanoLuc luciferase, called GeNL. b, c Fluorescent (b) and bioluminescent (c) DAF-16::GeNL before (0') and after (15') exposure to 37 °C heat stress. Inset shows animals at the same timepoints without external heat stress, but continuous external illumination. d Quantification of the nuclear/cytoplasmic DAF-16 ratio (Mean ± standard deviation (SD)) over 18 min of the experiment in the four tested conditions (representative for N = 5 animals). Scale bar = 40 μm.
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