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Fig. 1
Optimized bioluminescence microscopy.
a Photograph of the optimized LowLiteScope. b Bioluminescent (i, ×20 magnification) and epifluorescent (ii, ×63 magnification) images of cell expressing the indicated marker taken on the LowLiteScope (i) or a commercial epifluorescence (ii) microscope, respectively. Exposure times indicated in the top left of each image. Scale bar = 20 μm. c Bioluminescent images of an immobilized worm expressing a turquoise-enhanced Nanolantern (TeNL) in the body wall muscle at different exposure times. Insets show the intensity profile across the line indicated in the image. Scale bar = 50 μm.
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