ZFIN Image: Tian et al., 2022, Fig. 5

Image

Figure Caption

Fig. 5

D-2-HG inhibited mitochondrial respiration in liver organoids.

A Representative OCR experiments were analyzed in liver organoids treated with PBS, or 5 mM D-2-HG, using the Seahorse XF96 extracellular flux analyzer. Each group, n = 6–10. B Levels of mitochondrial respiratory function: basal respiration (OCR-BASAL), ATP production (OCR-ATP), maximal respiration (OCR-MMR), and spare respiratory capacity (OCR-SRC) in liver organoids. C Representative ECAR experiments performed on liver organoids treated with PBS or 5 mM D-2-HG. D Levels of glycolysis and maximal glycolytic capacity in liver organoids. E At 4 h after PBS or 5 mM D-2-HG exposure, the proteins of the EpCAM+ cells were extracted for western blotting (WB). The WB was used to determine the amounts of P-AMPK, AMPK, and ATPB (n = 3). ns, not significant, *P < 0.05, ***P < 0.001.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.