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Fig. 4
D-2-HG restrained the growth of liver organoids.
A Representative size and morphology of culture organoids treated with PBS, or 5 mM D-2-HG at the indicated time. B Quantification for the size of organoids in panel (A). C The relative expression of involved genes mRNA in the liver organoids with or without 5 mM D-2-HG exposure. D At 4 h after PBS or 5 mM D-2-HG exposure, the proteins of the EpCAM+ cells were extracted for western blotting (WB). Representative images of WB for CK19. Quantification of CK19 against GAPDH. ns, not significant, *P < 0.05.
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