|
FIGURE 6 gne KO larvae present disorganized slow and fast muscle fibers at 8 dpf. (A) Confocal imaging of F-actin stained gne KO muscle at 8 dpf. gne KO larvae show an abnormal Phalloidin staining pattern relative to the WT control at 8 dpf. Scale bar = 100 µm. (B) Confocal imaging of whole-mount immunostained larvae at 8 dpf by gne genotype. Zebrafish gne KO larvae exhibit impaired organization of the slow muscle fibers, stained with F59 antibody. Scale bar = 50 µm. (C) Confocal imaging of whole-mount immunostained larvae at 8 dpf by gne genotype. gne KO larvae exhibit a wavy pattern of the fast muscle fibers, and correct localization of Actin, Actinin, and Myomesin. Scale bar = 50 µm.