ZFIN Image: He et al., 2022, Figure 3

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Figure Caption

Figure 3

Anti-inflammatory effects of PEs on LPS-stimulated RAW264.7. Cells were pretreated with PEs at the concentrations of 50, 100 μg/mL for 1 h and exposed to 1 µg/mL LPS for another 24 h. After, IL-6 (a), IL-1β (b), and MMP-9 (c) production in the cell culture supernatant were detected by using an enzyme-linked immunosorbent assay (ELISA). The cells were collected and lysed for the measurement of the relative expression of genes involved in immune responses (IL-6 (d), IL-10 (e), IL-α (f), IL-1β (g), TNF-α (h), NOS (i), COX-2 (j), and PGE2 (k)) by qRT-PCR analysis (β-Actin expression was used as an internal control). Each experiment was performed in triplicate. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 compared to the group of LPS-treatment alone.

Acknowledgments

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