ZFIN Image: Kuhn et al., 2022, Fig. 2

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Figure Caption

Fig. 2

Localization of morphogens in cell-cell interfaces and extracellular cavities.

a Top: Overlay of the memGFP signal, the manually curated ROI separating cell-cell-interfaces (blue, I) and extracellular cavities (red, C); tracks assigned to interfaces (blue), cavities (red) and not assigned (green) are also shown. Bottom: Tracking-and-localization-microscopy (TALM) image showing the total number of localizations over 1000 frames or 11.7 s in each 2 × 2 pixel bin; scale bar: 5 µm. b Ratios of localization densities in cavities and interfaces calculated for each movie (for statistics see Supplementary Table ⁵). Values above 10 are not displayed (3.4% of all movies). p-values (Cyclops - Squint, Cyclops - Lefty1, Cyclops - Lefty2, Squint - Lefty1, Squint - Lefty2: <0.001; Lefty1 - Lefty2, Lefty1 - secHalo, Lefty2 -secHalo: >0.999) were calculated using the Kruskal-Wallis-Test. Solid black lines indicate the median values, dashed black lines the 0.25 and 0.75 quantiles. Source data are provided as a Source Data file for Fig. 2b.

Acknowledgments

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