FIGURE 5

FIGURE 5

Iron supplementation increases the proportion of functioning mitochondria. (A,B) Western blot analysis and quantitative results of LC3 expression in ZFL cells cultured under normoxia and hypoxia treated with or without FAC (2.5 mM), DFO (10 µM) and Fer-1 (2.5 µM) for 3 days. Actin as a loading control. (C,D) Western blot analysis and quantitative results of P62 expression in ZFL cells cultured under normoxia and hypoxia treated with or without FAC (2.5 mM), DFO (10 µM) and Fer-1 (2.5 µM) for 3 days. Actin as a loading control. (E) Representative images and quantitative results of Mitotraker red Dye to visualize mitochondrial mass in normoxia cell and cell treated with or without FAC (2.5 mM), DFO (10 µM) and Fer-1 (2.5 µM) under hypoxia stress for 3 days. (F) Representative images and quantitative results of Lysotraker red DND-99 Dye to visualize acid lysosomal in normoxia cell and cell treated with or without FAC (2.5 mM), DFO (10 µM) and Fer-1 (2.5 µM) under hypoxia stress for 3 days. Normoxia was used as a control group for significance analysis. Error bars, mean ± s.d., n = 3 (biological replicates).