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Fig. 3
ap3d1 gene expression is reduced overall in crasher mutants, but exon 14 of ap3d1 is overexpressed. (a) RNA-Seq data bar graph shows no reduction in any candidate gene in crasher mutants as compared to *AB to a log2 fold difference of 1. Log2 fold difference is the difference in mutant and *AB expression. However, ap3d1 expression is reduced the most. Negative values indicated reduced expression in crasher mutants as compared to *AB. A difference of 1 is considered of import and equivalent to a twofold difference. Significance testing was not performed. (b) ap3d1 expression is reduced in crasher mutants according to qRT-PCR (Student's t-test, t[4] = 2.94, p = 0.04*, n = 3). Error bars are standard deviation. (c) Exon 14 overexpression in ap3d1 line graph. The normalized reads ratio is defined as the normalized (transcripts per million) number of crasher reads at that exon divided by the normalized number of *AB reads at that exon.
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