Figure 4

Figure 4

Analysis of the quantity of movement of 5 dpf wfs1 mutant larvae during the noise cycle in the ASR test. (A) Sound protocol: the activity is measured for 45 min in a total dark (OFF) condition, with a training phase for 30 min silently, then three cycles of white sounds (90 dB) of 1 s each and 5 min interspersed. Quantity of movement per second for (B) the wfs1a^C825X and (F) wfs1b^W493X larvae according to the sound protocol. Relative quantity of movement during (C, G) the training phase [blue-dotted lines in (B, F) between 21 and 29 min]; (D, H) the baseline phase, period of 2 min before each sound (the averaged three baseline phases); (E, I) the three stimulations (the averaged sounds phases). Activity was expressed as % of associated controls. Error bars represent ± SEM calculated from three replicas. The number of animals is indicated below the columns. ^*P < 0.05; unpaired t-test.