ZFIN Image: Gong et al., 2022, Fig. 8

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Figure Caption

Fig. 8

LGP2 is essential for IFN response at the early stage of viral infection

(A) Stimulatory effects of zebrafish LGP2, MDA5, and RIG-I on fish IFN promoter activation by luciferase activity assays. CO cells seeded in 24-well plates were transfected with LGP2, MDA5, and RIG-I at increasing amounts (0, 5, 10, 200 ng) for 48 h. Western blots in (A and B) showed the expression of LGP2 in the transfected cells using anti-LGP2 Ab.

(B) mRNA expression comparison of cellular ifn gene by LGP2, MDA5, and RIG-I. EPC cells seeded in 6-well plates were transfected with LGP2, MDA5, and RIG-I at increasing amounts (0, 20, 40, 800 ng) for 48 h, followed by RT-PCR detection of lgp2 and ifn mRNA.

(C) RT-PCR detection of rig-I and mda5 mRNA in lgp2^+/+ and lgp2^lof/lof zebrafish larvae (6 dpf) or in spleen of lgp2^+/+ and lgp2^lof/lof zebrafish adults (60 dpf) following SVCV infection. Data were shown as mean ± SD (N = 3). P values were calculated using Student’s t test. ∗∗p < 0.01.

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Acknowledgments

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