ZFIN Image: Zheng et al., 2022, Fig. 1

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Figure Caption

Fig. 1

Generation of katnal2^-/- allele by CRISPR/Cas9 gene-editing technology. (A) Structure of zebrafish katnal2 gene. The protein includes an N-terminal LisH (Lis-homology) domain and a C-terminal AAA catalytic domain. A guide-RNA was designed to target a 23 bp region in exon 6. The katnal2^-/- allele contains a 2-base deletion and a 1-base insertion. The red color indicates the inserted nucleotides and the “-” indicates the deleted nucleotides. The mutation causes frameshift and early truncation of the protein prior to the AAA+ domain. (B) The expression pattern of katnal2 in the early embryonic development determined by in situ hybridization. (C) The expression level of katnal2 mRNA in katnal2^+/+ (pink) and katnal2^-/- (blue) alleles. Data are shown as mean ± S.E.M.; *** indicates p < 0.001.

Acknowledgments

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