Fig 2

Fig 2

Infection of 2 dpf AB larvae with 25 cfu of a 5:1 ratio of GFP:mCherry KN99 C. neoformans. Larvae were imaged until 8 dpf, or death (n = 3, in each repeat 7, 10 and 12 larvae were used) A Infection of AB wild-type larvae with 5:1 ratio of GFP:mCherry KN99 C. neoformans, at 0 dpi, 1 dpi, 2 dpi and 3 dpi A I Formation of cryptococcal masses at 1 dpi A II Final infection outcome B Infection of 2 dpf AB larvae with 25 cfu of a 5:1 ratio of GFP:mCherry KN99 C. neoformans. Larvae were imaged until 8 dpf, or death (n = 3, in each repeat 7, 10 and 12 larvae were used). A GFP majority infection outcome, mCherry infection outcome or a Mixed GFP and mCherry infection outcome (n = 3, 16 larvae) C Proportion of each overwhelming infection outcome observed, GFP, mCherry or mixed D Range of GFP:mChery C. neoformans injected into larvae at 2 hpi E Actual injected GFP:mCherry ratios for each overwhelming outcome (n = 3, +/- SEM, Man-Whitney t-test ns = not significant) F Inoculum ratio of GFP:mCherry, against final GFP:mCherry ratio at overwhelming infection stage (Linear regression R² = 0.0208, p<0.6081, n = 3, 16 larvae).