ZFIN Image: Zheng et al., 2022, FIGURE 2

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Figure Caption

FIGURE 2

Generation of the zebrafish nexmifa mutant using the CRISPR/Cas9 system. (A) Schematic showing the targeting site of the sgRNA in the third exon of nexmifa. (B) Mutation pattern of nexmifa-gRNA/cas9-injected embryos. Red letters represent the sgRNA sequence. Blue letters represent the additional 159 bp nucleotide sequence. (C) Schematic showing the predicted protein encoded by the mutated allele. Frameshift mutations resulted in truncated proteins. The gray rectangle indicates the wrong coded amino acid sequences.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.