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Fig. 6 Cdh2 endocytosis of endodermal cells is impaired in gpc4−/− embryos. (A-B′) Confocal images of Z-projections of the indicated embryos (high magnification and resolution) showing Cdh2 expression in endodermal cells, as detected by immunofluorescence. Arrowheads indicate cytoplasmic Cdh2-expressing puncta. (C) Average number of cytoplasmic Cdh2 puncta per cell in the indicated embryos from three experiments, represented by different colors. (D-E″) Confocal images of Z-projections showing the expression of Cdh2 and Eea-1 (detected by immunofluorescence) in endodermal cells from the indicated embryos. White arrowheads indicate Cdh2 and Eea-1 colocalized puncta. (F-G′) Single z-plane confocal images showing Eea-1 expression (detected by immunofluorescence) in endodermal cells from the indicated embryos. White arrowheads indicate Eea-1 expression. (H) Average number of cytoplasmic Eea-1-expressing puncta per cell in the indicated embryos from two separate experiments, represented by different colors. Data are mean±s.e.m. ****P<0.0001 (unpaired, two-tailed Student's t-test).