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Fig. 4 Vangl2 asymmetrical enrichment in endodermal cells is impaired in gpc4−/− embryos. (A) Schematic showing endoderm transplantation. (B-C′) Confocal images showing GFP-Vangl2 distribution in transplanted control endodermal cells in control embryos (B,B′) and transplanted gpc4−/− endodermal cells in gpc4−/− embryos (C,C′). Yellow arrowheads indicate ML boundaries of endodermal cells. (D) Percentage of donor-endodermal cells expressing GFP-Vangl2 at the anterior ML cell boundary in the indicated host embryos. The number of embryos and cells analyzed is indicated. (E-F′) Confocal images showing GFP-Vangl2 localization in endodermal cells in the indicated embryos. Yellow and cyan arrowheads indicate ML and AP boundaries of endodermal cells, respectively. (G) Maximum intensity of GFP-Vangl2 on ML (yellow) and AP (cyan) boundaries of endodermal cells in the indicated embryos. The number of embryos and cells analyzed is indicated. Data are mean±s.e.m. n.s., not significant, P>0.05; ****P<0.0001 (unpaired, two-tailed Student's t-test).