ZFIN Image: Leggieri et al., 2022, FIGURE 5

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Figure Caption

FIGURE 5

Quantification of dopaminergic gene expression. (A) Expression levels of drd2a, drd2b, drd1, drd3, drd4a, drd4b, drd5, dat, and dbh in 5 days post fertilization zebrafish larvae (ankk1^+/+, ankk1^+/27ins, and ankk1^27ins/27ins) measured by qPCR. Each dot represents a pool of larvae (n_total = 80 larvae: 5 samples consisting of 16 larvae for ankk1^+/+ and ankk1^+/27ins, and 4 samples consisting of 16 larvae for each sample (ankk1^27ins/27ins). (B) Expression levels of drd2a and drd2b in adult zebrafish whole brains. Each dot represents a single brain. Data are shown in box-whiskers plot (5–95 percentile). Legend: *p < 0.05 versus ankk1^+/+; **p < 0.01 versus ankk1^+/+; ***p < 0.0001 versus ankk1^+/+; +p < 0.05 versus corresponding ankk1^+/27ins; ++p < 0.01 versus corresponding ankk1^+/27ins. Statistics for gene expression data and P value adjustment are provided in Supplementary Tables 2, 3.

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Acknowledgments

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