ZFIN Image: Karuppan et al., 2021, Figure 3

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Figure Caption

Figure 3

Validation of CNTN–amyloid interactions by biolayer interferometry. The E1 domains of APP, APLP1, and APLP2 were biotinylated in a 1:1 M ratio, immobilized onto streptavidin sensors, and titrated against varying concentrations of the FN1–FN3 region of selected CNTN proteins. One representative experiment for each series is shown in A–C, whereas a summary of the affinities measured for at least three biological replicates is shown below each curve. The plots were normalized to the maximal response. APLP1, amyloid beta precursor like protein 1; APLP2, amyloid beta precursor like protein 2; APP, amyloid precursor protein; CNTN, contactin; FN1, first FN repeat; FN3, third FN repeat.

Acknowledgments

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