ZFIN Image: Huang et al., 2021, Fig. 2

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Figure Caption

Fig. 2

Injury-induced ISNs are indispensable for axon regrowth and locomotion recovery.

a Metronidazole (MTZ) treatment protocols used to genetically ablate mCherry⁺ ISNs after SCI using Tg(tph2:Gal4;UAS:nfsB-mCherry) line. b Immunohistochemistry images show the distribution of mCherry⁺ ISNs and Dextran 488⁺ retrogradely labeled axon-regrown spinal interneurons in 1-cut SCI fish with/without MTZ treatment. Injury sites are indicated by white arrows. c–g Ablation of ISNs by MTZ treatment prevents axon regrowth and locomotion recovery after SCI. Quantification of mCherry⁺ ISNs numbers in the region of 500 μm around the injury site (c); free-swimming speed (d); maximum swimming speed (e); retrogradely labeled axon-regrown spinal interneuron numbers (f) and regrown axon density (g) after SCI with/without MTZ treatment. h Immunohistochemistry images show the distribution of mCherry⁺ ISNs and Dextran 488⁺ retrogradely labeled axon-regrown spinal interneurons in two-cut SCI fish with/without MTZ treatment after the second injury. i–m Effect of ISNs ablation in 2-cut SCI model. Mean data of mCherry⁺ ISNs numbers in a 500 μm region covering the secondary injury site (i); free-swimming speed (j); maximum speed (k); retrogradely labeled axon-regrown spinal interneuron numbers (l) and regrown axon density (m) after the second injury with/without MTZ treatment. All data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, significant difference. For detailed statistics, see Supplementary Table ¹.

Acknowledgments

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