ZFIN Image: Matrone et al., 2021, Fig. 6

Image

Figure Caption

Fig. 6

Modulation of Kdm1a S-nitrosylation during tailfin regeneration.

A Kdm1a mRNA C334A was generated by site-directed mutagenesis, replacing the aa Cys334 with Ala. B–D Zebrafish embryos injected with kdm1a morpholino (Mo), or co-injected with kdm1a Mo with kdm1a mRNA C334A or wild type. B Western blotting and semi-quantitative analysis showed the effective knockdown and rescue of kdm1a following the different treatments. β-tubulin was used as loading control. C, D Images and dot plot of tailfin regeneration following kdm1a modulation (p values vs. control). Scale bar measures 100 μm. E Working model. Tissue injury promotes the S-nitrosylation of the Cys334 of Kdm1a. S-nitrosylated Kdm1a detaches from the CoRest complex and loses its demethylase activity on H3K4. One-way ANOVA followed by Bonferroni’s multiple comparisons test, p values indicate comparisons vs. control. N = 3 biological replicates. Data are presented as mean values +/− SEM.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.