FIGURE 6

FIGURE 6

LGK974 treatment suppresses shortened body length and chondrocyte defect caused by of COG4^p.G516G expression in zebrafish. (A) Scheme of the LGK974 treatment procedure. (B) Graphs show the measured body length of each group at 4 dpf. The data are presented as mean ± SEM. An unpaired two-tailed t-test was used. ^∗∗∗p < 0.001; ^∗∗p < 0.01; ^∗p < 0.05; ns, not significant. (C) Ventral view of representative Meckel’s cartilage of control and COG4^p.G516G-injected embryos with or without LGK974 treatment at 4 dpf following WGA staining and imaged by a confocal microscope. (D) Graphs show the length-to-width ratio of chondrocytes in Meckel’s cartilage in (C) and two more representative Meckel’s cartilage images of each group. The data are presented as mean ± SEM. One-way ANOVA with Tukey’s multiple comparison tests was applied. ^****p < 0.0001; ^∗∗p < 0.01; ns, not significant. Experiments were performed in two biological replicates with similar results.