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Figure 4
zAvatars generated from different histological pancreatobiliary tumors. For all examples, cells were extracted from the surgical resected sample, injected in 2dpf embryos, and tested for chemotherapy schemes. At 3 dpi and 2 days post-treatment (2 dpT), zAvatars were sacrificed and fixed, subjected to whole-mount immunofluorescence and imaged by confocal fluorescent microscopy. (a) Confocal image representative of a zAvatar injected with PDAC cells (labeled in magenta) in a Tg (fli1:eGFP) zebrafish background (labeled in green) at 3 dpi. (b) Percentage of engraftment of the different zAvatars; the total number of xenografts analyzed at the end of the assay is depicted in the figure. (c) Quantification of the apoptotic index (fold induction of activated caspase-3). (d) Quantification of the tumor size (fold change of the number of tumor cells). All results are expressed as AVG ± SEM. Statistical analysis was performed using Mann–Whitney test. Statistical results: (ns) > 0.05, * p ≤ 0.05, ** p ≤ 0.01. Each dot represents one zAvatar. (e,g,i,k) Hematoxylin and eosin staining of Patient #1, #2, #4, and #5 pathology samples, respectively. Scale bars represent 100 µm. (f,h,j,l) Representative confocal images of control and treatment zAvatars #1, #2, #4, and #5, respectively. Note that only zAvatar #5 is a Tg (fli1:eGFP), where zebrafish blood vessels can be detected in green. Scale bars represent 50 µm. For all images, tumor cells are labeled in white, activated caspase-3 in green, human mitochondria in red, and DAPI in blue. All images are anterior to the left, posterior to right, dorsal up, and ventral down.