ZFIN Image: Gao et al., 2021, Fig 1

Image

Figure Caption

Fig 1 The establishment of a zebrafish <italic toggle='yes'>irf2bp2a</italic> knockout line.

(A) Schematic representation of Cas9 target site in the first exon of zebrafish irf2bp2a. The deleted nucleotides in the mutant gene are marked by hyphens. (B) Schematic representation of wild type (493 amino acids) and mutant Irf2bp2a proteins (122 amino acids). The site where the frameshift was introduced is marked by triangles. (C) Western blot analysis of FLAG-tagged wild type and mutant Irf2bp2a proteins expressed in HEK293 cells. (D) Immunofluorescence analysis of FLAG-tagged wild type (top panel) and mutant Irf2bp2a (bottom panel) proteins, demonstrating that the truncated protein lost its nuclear localization. Scale bar, 10 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.