ZFIN Image: Wen et al., 2021, Fig 4

Image

Figure Caption

Fig 4 <italic toggle='yes'>Klf6a</italic> regulates EC nucleus migration in CV pruning.

(A) Time-lapse live imaging of Tg(fli1a:nEGFP;kdrl:mCherry) zebrafish embryos shows EC nucleus migration in CV pruning in control and klf6a morphant. The arrow shows the direction of the blood flow. Colored circles indicate EC nuclei. Scale bar: 10 μm. (B) The direction of EC migration within the regressing vessel. The direction is classified into three types: with the flow, static, and against the flow. A total of 10 vascular loops with 14 EC nuclei and 14 vascular loops with 21 EC nuclei were calculated in the control group and klf6a morphant group, respectively. P = 0.0058. Unpaired two-tailed chi-square test. (C) and (D) Efficiency of siKLF6 in human umbilical vein endothelial cells (HUVECs) at the mRNA (P = 0.00087) and protein (P = 0.0003) levels. (E) Wound healing of siCTR-transfected and siKLF6-transfected HUVECs: 6 h, P = 0.0013; 12 h, P = 0.0020. (C) to (E) Student’s unpaired two-tailed t test. *P < 0.05, **P < 0.01, ***P < 0.001.

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Acknowledgments

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