ZFIN Image: Li et al., 2021, FIGURE 2

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Figure Caption

FIGURE 2

Generation of syne2b mutation by CRISPR/Cas9-mediated genome editing. (A) Organization of the zebrafish syne2b locus. Shown are the sgRNA targeting sites (sg1 and sg2) and the deleted intervening region. (B) Genotyping of syne2b mutation. The sgRNA recognition sequences are green colored, and the PAM regions are shown in blue. Arrows indicate the positions of deletion in exons 120 and 129. Nucleotides inserted as a result of DNA repair are underlined. (C) Syne2b protein domains. Arrow indicates the position after which truncation occurs in the translated Syne2b protein. (D) Alignment of the C-terminal regions from wild-type and truncated Syne2b proteins. Residues translated after frameshift are indicated in red.

Acknowledgments

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