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Fig. 3 Dose–response curves of selected agonist-receptor pairs. The cDNA constructs of the bitter taste receptors of coelacanth (lcT2R#) and zebrafish (drT2R#) were transiently transfected into HEK 293T-Gα16gust44 cells and subsequently subjected to calcium mobilization assays using an automated fluorometric imaging plate reader (FLIPRtetra). Cells transfected with an empty expression vector served as negative controls (mock). The relative changes in fluorescence (ΔF/F) are plotted on the y axis (means ± SD, n = 4), the applied compound concentrations on the logarithmically scaled x axis.
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