Figure 1

Figure 1

Phylogenetic analysis and embryonic expression of Stag paralogues. (A) Phylogenetic analysis of predicted protein sequences using the maximum likelihood approach. The accession numbers for the protein sequences used in this analysis are listed in Supplementary Table 1. (B) Whole-mount in situ hybridization of stag1a, stag1b, stag2a, and stag2b during early embryogenesis. Lateral views are shown, anterior to the left. Scale bars are 50 μm for embryos at 12 and 15 hpf and 100 μm for embryos at 24 hpf. (C,D) mRNA expression of stag paralogues at the indicated time points during (C) early embryogenesis and (D) late embryogenesis. Each data point represents mRNA isolated from a pool of 30 embryos. Graphs are mean +/- one standard deviation. Expression was normalized to the reference genes, b-actin and rpl13a (Supplementary Figure 1A).