Figure 5

Figure 5

TCM pharmacological validation of heg1^∆25 zebrafish mutant. (A) Phenotype observation and the relative movement of RBCs in PVC analysis of heg1^∆25 embryos treated with four TCM herbs. (B) The optimal concentrations of the four drugs, as indicated as Radix astragali 1300 mg/L, Salviae miltiorrhiza 2000 mg/L, Hirudo 400 mg/L, and Myrrha 100 mg/L, respectively. N = 40 embryos for each concentrations. (C) Comparison of heart rate of zebrafish embryos in each group. (D) Comparison of SV-BA distance of zebrafish embryos in each group. (E) Comparison of pericardial area of zebrafish embryos in each group. Data are represented as mean ± SE from three independent experiments, * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s t-test).