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Fig. 4 Cyp19a1a promotes bipotential and female development by inhibition of dmrt1. (A-D″) Buc (A-D), Vasa (A′-D′) and Buc/Vasa/DAPI merged (A″-D″) staining in representative slices from individual Z-series allows visualization of gonocyte development and sex-specific differentiation in d45 juvenile zebrafish, including female cyp19a1auc38/+;dmrt1uc27/uc27 (HM) (n=3/3) (A), male cyp19a1auc38/uc38;dmrt1uc27/+ (MH) (n=5/5) (B) and cyp19a1auc38/uc38;dmrt1uc27/uc27 (MM) gonads that were either male (n=1/4) with male germ cells (C) or female (n=3/4) with oocytes (D), as shown by expression of the oocyte marker Buc (white) and indicated with white arrowheads.