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Fig. 2 Rbpms2 is required for female gonad differentiation even in the absence of an essential male fate regulator. (A-F″) Vasa (A-F), DAPI (A′-F′) and merged (A″-F″) staining. Representative slice from a Z-stack reveals gonad development of d28 juvenile zebrafish, including female (n=1/3) (A) or male (n=2/3) (B) triple heterozygote (HHH) siblings. All rbpms2DM (MMH) gonads recovered were either intersex (n=2/3) (C) or male (n=1/3) (D). The dmrt1 mutant siblings (HHM) were female (n=3/3) (E) and triple mutant siblings (MMM) were intersex (n=3/3) (F). Oocyte-like cells are outlined in white dashed lines and testis-like cells in orange dashed line.