ZFIN Image: Grivas et al., 2020, Figure 6

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Figure 6

Impaired cardiac function and stiffer heart tissue in caveolae-deprived cav1^cn100 mutants. (a) Quantification of ejection fraction in cav1^+/+ and cav1^cn100 hearts. n_WT = 22, n_cn100 = 20. (b) Heart rate measurements in cav1^+/+ and cav1^cn100 animals. n_WT = 23, n_cn100 = 20. t-test, *P < 0.05. (c) AFM set-up. Cartoon made with Adobe Illustrator CC2018 (www.adobe.com). (d, e) Images of the cantilever (arrowhead), the laser beam on top of the cantilever (dashed circle, also in c) and the ventricle (asterisk). Images were taken with an inverted optical microscope (AXIO Observer D1; Carl Zeiss, Germany, see Methods). (f) Biomechanical characterization of cav1^+/+ and cav1^cn100 ventricles as measured by AFM-force spectroscopy and expressed in Young’s moduli. n_WT = 9, n_cn100 = 8, t-test, ***P < 0.001. (g) Force-distance graph for indentation and retraction of the cantilever over the ventricular surface.

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Acknowledgments

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